Species of fruit flies (Tephritidae) were surveyed in a transect of the Amazonian Rainforest in the district of Clevelândia do Norte, municipality of Oiapoque, state of Amapá, Brazil. This study describes the diversity of fruit fly species sampled inside this transect (2.2 linear km). Eleven McPhail traps were serviced weekly from June 2002 to June 2003. A total of 55 samples were obtained, totaling 125 specimens of Tephritidae. Anastrepha Schiner, 1868 was the most abundant and diverse genus, with 18 species caught. Two new species, A. oiapoquensis Norrbom & Uchôa, 2011 and A. siculigera Norrbom & Uchôa, 2011, were discovered in this survey. Anastrepha coronilli Carrejo & González, 1993 (n = 40) was the most abundant species. Six species, Anastrepha amita Zucchi, 1979, A. duckei Lima, 1934, A. flavipennis Greene, 1934, A. minensis Lima, 1937, A. pseudoparallela (Loew, 1873), and A. submunda Lima, 1937, are recorded for the first time from Amapá. Three of them, A. minensis, A. pseudoparallela, and A. submunda, are reported for the first time from northern Brazil. This paper updates to 36 the number of frugivorous species of Tephritidae in Amapá.
